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Puromycin invitrogen

WebMedium containing 1 μg/mL puromycin was used to select the stable cells. Transient-Transfected Cell Construction. The miR-135b, miR-186-5p and miR-218-5p mimics duplexes were synthesized by Genepharma (Shanghai, China). HEK293T cells were transfected with miRNAs mimics using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the ... WebJul 1, 2024 · Additionally, BV2 cells stably expressing genes were maintained in DMEM supplemented with 10% FBS and 5 μg/ml puromycin (Invitrogen). BV2 cells were plated at …

Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem 540222

WebDec 30, 2024 · Puromycin dihydrochloride is a aminonuclease antibiotic that inhibits protein synthesis. Puromycin is used for selection and maintenance of cell lines expressing a transfected pac gene (S. alboniger), whose … WebPuromycin Invitrogen A11138-03 10mg/ml 10ug/ml Trypsin-EDTA Invitrogen 25200-056 . Equipment : Flask T75 (Corning #430641U), Centrifuge. Medium . Growth Medium . L ... 6. … suncorp offices sunshine coast https://maidaroma.com

Determining the appropriate concentration of puromycin for cell …

WebThe pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse genes. Addgene is … WebPuromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene. … Web10 rows · 嘌呤霉素. 嘌呤霉素（Puromycin）是一种由白黑链霉菌产生的氨基苷类抗生素。. 其可以打乱核糖体上的肽转运，造成翻译过程中不成熟链终止，从而抑制蛋白质合成。. … suncorp pds landlord

K562 cell culture and formaldehyde cross-linking - ENCODE

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Puromycin invitrogen

Puromycin-based purification of cells with high expression of the ...

WebPuromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It is a very common antibiotic routinely used by scientists in biomedical research to select cells … WebAug 22, 2010 · A gene conferring puromycin resistance can be used for antibiotic selection in C. elegans and C. briggsae. ... combinations using the three-way Gateway system …

Puromycin invitrogen

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WebThe objective of the following work was to study the apoptosis inducing effect of puromycin in MCF-7 breast cancer cells and compare this effect with cycloheximide and emetine, 2 … WebApr 7, 2024 · Perurena et al. show that PDACs are resistant to MEK inhibitors because they trigger the formation of USP9X:Mcl-1 complexes, thereby acutely stabilizing Mcl-1 and inhibiting apoptosis. Resistance can be overcome by targeting Mcl-1 with direct inhibitors or by suppressing Mcl-1 transcription with CDK inhibitors, revealing new therapeutic …

WebInvitrogen™ Custom DNA Oligos Tool; Invitrogen™ Custom RNAi Tool; Assay Tools. ... Formula: C22H29N7O5 • 2HCl, Purity: ≥ 98% (HPLC), Molecular weight: 544.3, Endotoxin … WebPlasmid pcDNA4-TO-Puromycin-mVenus-MAP from Dr. Dannel McCollum's lab is published in Mol Cell Proteomics. 2012 Aug;11(8):501-11. doi: 10.1074/mcp.O111.016246. ... which …

Web引用和文献. 嘌呤霉素盐酸盐是一种由 白黑链霉菌 产生的氨基苷类抗生素。. 嘌呤霉素的作用机制为抑制原核和真核核糖体上的肽基转移；耐药性取决于 pac 基因的表达水平。. 嘌呤 … Webpuromycin resistance gene RI random integration RMCE recombinase‐mediated cassette exchange UCOE ubiquitous chromatin ... Invitrogen). After 2–3 days of transfection, …

WebInvitrogen Supplier A Supplier B The Geneticin Advantage; Purity (HPLC) >90–93%. 66–75% . 65–82%. Significantly higher purity. Potency claimed ... while cells in suspension are …

WebDay 1: Plate target cells in ten 6 cm plates and grow at 37° C, 5% CO 2 overnight. Day 2: The target cells should be approximately 80-90% confluent. Dilute puromycin in the preferred … suncorp offices sydneyWebPuromycin is a broad spectrum antibiotic produced by Streptomyces alboniger. It is an analog of the 3′ end of aminoacyl-tRNA. 1 2 It is an inhibitor of peptidyl transferase and is … suncorp order new cardWebSerum (FBS), 1% Pen-Strep (Invitrogen 15140-163), 2 µg/ml puromycin (Invitrogen A11138-03) and 3 µg/ml doxycycline (Clontech 631311). General protocol notes: • Incubate all … suncorp phone nos