WebSep 1, 2014 · A slurry of Toyopearl DEAE-650M was packed in a column (20 × 2.5 cm), then equilibrated with 5 column volume (CV) of equilibrating buffer and 20 mM Tris-HCl buffer (pH 9.0). Afterwards, 100 mg of lyophilized hydrolysates that had been through the 0.45 μm filter film was dissolved in 10 mL of the same buffer (pH 9.0) and loaded on the … WebProcess and Economic Evaluation of the Extraction and Purification of Recombinant β-Glucuronidase from Transgenic Corn
Ion Exchange Chromatography
WebThe AAR was purified to homogeneity from the crude extract of the E. coli transformant by two steps: heat treatment and Butyl-Toyopearl column chromatography. Bioreactors for the production of optically active amino acids were constructed with DEAE-Toyopearl-immobilized AAR and D- or L-aminoacylase. Webmethod of analyzing a monomer of a recombinant extracellular matrix protein by size exclusion chromatography: 申请号: ep22174158.0: 申请日: 2024-12-29: 公开(公告)号: ep4070869a1: 公开(公告)日 first choice wsale pension
Deae Toyopearl 650m Column Tosoh Corporation Bioz
WebApr 16, 2015 · Methods for producing prothrombin complex concentrate (PPSB) by anion-exchange chromatography in batch mode using various sorbents such as DEAE- and QAE-Sephadex A-50 were examined. It was established that the yield and specific activity of FII, FVII, FIX, and FX depended on the mass of resin used for chromatography. New … WebMay 24, 2024 · Elution profile of the U. linza seaweed extract on DEAE-Toyopearl column chromatography. The seaweed extract (5%, 100 ml) was subjected to the column (2.0 × 20 cm) equilibrated with 20 mM Tris-HCl (pH 7.5). After washing the column with the same buffer, elution was made with a linear gradient from 0 to 1.0 M NaCl in the same buffer WebTOYOPEARL DEAE-650 can be used for high throughput capture, intermediate purification, and polishing process steps. It is frequently used in plasma fractionation and for the … firstchoice wsale personal super account abn